Citrus tristeza virus infection induces the accumulation of viral small RNAs (21-24-nt) mapping preferentially at the 3'-terminal region of the genomic RNA and affects the host small RNA profile.
Identifieur interne : 001775 ( Main/Exploration ); précédent : 001774; suivant : 001776Citrus tristeza virus infection induces the accumulation of viral small RNAs (21-24-nt) mapping preferentially at the 3'-terminal region of the genomic RNA and affects the host small RNA profile.
Auteurs : Susana Ruiz-Ruiz [Espagne] ; Beatriz Navarro ; Andreas Gisel ; Leandro Pe A ; Luis Navarro ; Pedro Moreno ; Francesco Di Serio ; Ricardo FloresSource :
- Plant molecular biology [ 1573-5028 ] ; 2011.
English descriptors
- KwdEn :
- 3' Untranslated Regions (genetics), 3' Untranslated Regions (physiology), Blotting, Northern, Citrus aurantiifolia (genetics), Citrus aurantiifolia (virology), Citrus sinensis (genetics), Citrus sinensis (virology), Closterovirus (genetics), Closterovirus (physiology), Plant Diseases (genetics), Plant Diseases (virology), Plant Immunity (genetics), RNA Interference (physiology), RNA, Plant (genetics), RNA, Plant (physiology), RNA, Small Interfering (genetics), RNA, Small Interfering (physiology), RNA, Viral (genetics), RNA, Viral (physiology), Reverse Transcriptase Polymerase Chain Reaction.
- MESH :
- chemical , genetics : 3' Untranslated Regions, RNA, Plant, RNA, Small Interfering, RNA, Viral.
- chemical , physiology : 3' Untranslated Regions, RNA, Plant, RNA, Small Interfering, RNA, Viral.
- genetics : Citrus aurantiifolia, Citrus sinensis, Closterovirus, Plant Diseases, Plant Immunity.
- physiology : Closterovirus, RNA Interference.
- virology : Citrus aurantiifolia, Citrus sinensis, Plant Diseases.
- Blotting, Northern, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
To get an insight into the host RNA silencing defense induced by Citrus tristeza virus (CTV) and into the counter defensive reaction mediated by its three silencing suppressors (p25, p20 and p23), we have examined by deep sequencing (Solexa-Illumina) the small RNAs (sRNAs) in three virus-host combinations. Our data show that CTV sRNAs: (i) represent more than 50% of the total sRNAs in Mexican lime and sweet orange (where CTV reaches relatively high titers), but only 3.5% in sour orange (where the CTV titer is significantly lower), (ii) are predominantly of 21-22-nt, with a biased distribution of their 5' nucleotide and with those of (+) polarity accumulating in a moderate excess, and (iii) derive from essentially all the CTV genome (ca. 20 kb), as revealed by its complete reconstruction from viral sRNA contigs, but adopt an asymmetric distribution with a prominent hotspot covering approximately the 3'-terminal 2,500 nt. These results suggest that the citrus homologues of Dicer-like (DCL) 4 and 2 most likely mediate the genesis of the 21 and 22 nt CTV sRNAs, respectively, and show that both ribonucleases act not only on the genomic RNA but also on the 3' co-terminal subgenomic RNAs and, particularly, on their double-stranded forms. The plant sRNA profile, very similar and dominated by the 24-nt sRNAs in the three mock-inoculated controls, was minimally affected by CTV infection in sour orange, but exhibited a significant reduction of the 24-nt sRNAs in Mexican lime and sweet orange. We have also identified novel citrus miRNAs and determined how CTV influences their accumulation.
DOI: 10.1007/s11103-011-9754-4
PubMed: 21327514
Affiliations:
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uniqKey="Navarro B" first="Beatriz" last="Navarro">Beatriz Navarro</name></author><author><name sortKey="Gisel, Andreas" sort="Gisel, Andreas" uniqKey="Gisel A" first="Andreas" last="Gisel">Andreas Gisel</name></author><author><name sortKey="Pe A, Leandro" sort="Pe A, Leandro" uniqKey="Pe A L" first="Leandro" last="Pe A">Leandro Pe A</name></author><author><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name></author><author><name sortKey="Moreno, Pedro" sort="Moreno, Pedro" uniqKey="Moreno P" first="Pedro" last="Moreno">Pedro Moreno</name></author><author><name sortKey="Di Serio, Francesco" sort="Di Serio, Francesco" uniqKey="Di Serio F" first="Francesco" last="Di Serio">Francesco Di Serio</name></author><author><name sortKey="Flores, Ricardo" sort="Flores, Ricardo" uniqKey="Flores R" first="Ricardo" last="Flores">Ricardo Flores</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date 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last="Ruiz-Ruiz">Susana Ruiz-Ruiz</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, Valencia, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Navarro, Beatriz" sort="Navarro, Beatriz" uniqKey="Navarro B" first="Beatriz" last="Navarro">Beatriz Navarro</name></author><author><name sortKey="Gisel, Andreas" sort="Gisel, Andreas" uniqKey="Gisel A" first="Andreas" last="Gisel">Andreas Gisel</name></author><author><name sortKey="Pe A, Leandro" sort="Pe A, Leandro" uniqKey="Pe A L" first="Leandro" last="Pe A">Leandro Pe A</name></author><author><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name></author><author><name sortKey="Moreno, Pedro" sort="Moreno, Pedro" uniqKey="Moreno P" first="Pedro" last="Moreno">Pedro Moreno</name></author><author><name sortKey="Di Serio, Francesco" sort="Di Serio, Francesco" uniqKey="Di Serio F" first="Francesco" last="Di Serio">Francesco Di Serio</name></author><author><name sortKey="Flores, Ricardo" sort="Flores, Ricardo" uniqKey="Flores R" first="Ricardo" last="Flores">Ricardo Flores</name></author></analytic><series><title level="j">Plant molecular biology</title><idno type="eISSN">1573-5028</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3' Untranslated Regions (genetics)</term><term>3' Untranslated Regions (physiology)</term><term>Blotting, Northern</term><term>Citrus aurantiifolia (genetics)</term><term>Citrus aurantiifolia (virology)</term><term>Citrus sinensis (genetics)</term><term>Citrus sinensis (virology)</term><term>Closterovirus (genetics)</term><term>Closterovirus (physiology)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (virology)</term><term>Plant Immunity (genetics)</term><term>RNA Interference (physiology)</term><term>RNA, Plant (genetics)</term><term>RNA, Plant (physiology)</term><term>RNA, Small Interfering (genetics)</term><term>RNA, Small Interfering (physiology)</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (physiology)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>3' Untranslated Regions</term><term>RNA, Plant</term><term>RNA, Small Interfering</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>3' Untranslated Regions</term><term>RNA, Plant</term><term>RNA, Small Interfering</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus aurantiifolia</term><term>Citrus sinensis</term><term>Closterovirus</term><term>Plant Diseases</term><term>Plant Immunity</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Closterovirus</term><term>RNA Interference</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Citrus aurantiifolia</term><term>Citrus sinensis</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Northern</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To get an insight into the host RNA silencing defense induced by Citrus tristeza virus (CTV) and into the counter defensive reaction mediated by its three silencing suppressors (p25, p20 and p23), we have examined by deep sequencing (Solexa-Illumina) the small RNAs (sRNAs) in three virus-host combinations. Our data show that CTV sRNAs: (i) represent more than 50% of the total sRNAs in Mexican lime and sweet orange (where CTV reaches relatively high titers), but only 3.5% in sour orange (where the CTV titer is significantly lower), (ii) are predominantly of 21-22-nt, with a biased distribution of their 5' nucleotide and with those of (+) polarity accumulating in a moderate excess, and (iii) derive from essentially all the CTV genome (ca. 20 kb), as revealed by its complete reconstruction from viral sRNA contigs, but adopt an asymmetric distribution with a prominent hotspot covering approximately the 3'-terminal 2,500 nt. These results suggest that the citrus homologues of Dicer-like (DCL) 4 and 2 most likely mediate the genesis of the 21 and 22 nt CTV sRNAs, respectively, and show that both ribonucleases act not only on the genomic RNA but also on the 3' co-terminal subgenomic RNAs and, particularly, on their double-stranded forms. The plant sRNA profile, very similar and dominated by the 24-nt sRNAs in the three mock-inoculated controls, was minimally affected by CTV infection in sour orange, but exhibited a significant reduction of the 24-nt sRNAs in Mexican lime and sweet orange. We have also identified novel citrus miRNAs and determined how CTV influences their accumulation.</div></front></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><noCountry><name sortKey="Di Serio, Francesco" sort="Di Serio, Francesco" uniqKey="Di Serio F" first="Francesco" last="Di Serio">Francesco Di Serio</name><name sortKey="Flores, Ricardo" sort="Flores, Ricardo" uniqKey="Flores R" first="Ricardo" last="Flores">Ricardo Flores</name><name sortKey="Gisel, Andreas" sort="Gisel, Andreas" uniqKey="Gisel A" first="Andreas" last="Gisel">Andreas Gisel</name><name sortKey="Moreno, Pedro" sort="Moreno, Pedro" uniqKey="Moreno P" first="Pedro" last="Moreno">Pedro Moreno</name><name sortKey="Navarro, Beatriz" sort="Navarro, Beatriz" uniqKey="Navarro B" first="Beatriz" last="Navarro">Beatriz Navarro</name><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name><name sortKey="Pe A, Leandro" sort="Pe A, Leandro" uniqKey="Pe A L" first="Leandro" last="Pe A">Leandro Pe A</name></noCountry><country name="Espagne"><noRegion><name sortKey="Ruiz Ruiz, Susana" sort="Ruiz Ruiz, Susana" uniqKey="Ruiz Ruiz S" first="Susana" last="Ruiz-Ruiz">Susana Ruiz-Ruiz</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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